stat2 (Proteintech)
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Structured Review
Proteintech
stat2
Stat2, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+stat2/STAT2+Antibody/pmc13026504-59-4-18
Average 94 stars, based on 81 article reviews
Stat2, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+stat2/STAT2+Antibody/pmc13026504-59-4-18
Average 94 stars, based on 81 article reviews
stat2 - by Bioz Stars,
2026-09
94/100 stars
Images
1) Product Images from "Conditional Stat2 Knockout Mice as a Platform for Modeling Human Diseases"
Article Title: Conditional Stat2 Knockout Mice as a Platform for Modeling Human Diseases
Journal: Immuno
doi: 10.3390/immuno6010007
Figure Legend Snippet: Generation of conditional Stat2 KO mice. ( a ) Targeting strategy used to construct Stat2 fl/fl mice with exons 5 through 8 flanked with loxP sites. E represents exons with their corresponding number. Brown boxes depict exons in wild type allele and green boxes depict exons after successful integration of targeting vector. ( b ) Confirmation of loxP sites integration in the Stat2 gene by Southern blot analysis (9.8 kb DNA fragment). ( c ) Genotyping of mouse-tail DNA by PCR confirms deletion of Stat2 floxed allele after breeding with CMV-Cre mouse.
Techniques Used: Construct, Plasmid Preparation, Southern Blot
Figure Legend Snippet: Impaired IFN-I signaling after conditional deletion of Stat2 by CMV-Cre—mediated recombination. ( a ) Loss of Stat2 gene expression validated in four different organs by qRT-PCR analysis. ( b ) Western blot analyses performed on several tissues confirm global Stat2 deletion ( Stat2 Δ / Δ ) by ubiquitous CMV-Cre recombinase. ( c ) Splenocytes of the indicated genotypes were left untreated or treated with IFN-β for 6 h, and ISG expression was determined by qRT-PCR. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001. Data are presented as SEM of three independent experiments. n.s; not significant.
Techniques Used: Gene Expression, Quantitative RT-PCR, Western Blot, Expressing
Figure Legend Snippet: Stat2 Δ / Δ mice show enhanced tumor growth. ( a ) B16-F1 or ( b ) EL4 tumor cells were injected subcutaneously in WT, Stat2KO , Stat2fl/fl and Stat2 Δ/Δ . Values are shown as mean tumor volume determined over 20 days. Representative images of individual tumors are shown. *, p ≤ 0.05; **, p ≤ 0.01; and ***, p ≤ 0.001. n = 6–8 animals/group.
Techniques Used: Injection
Figure Legend Snippet: Stat2 Δ / Δ fibroblasts have impaired antiviral response to type I IFNs. ( a ) Lung fibroblasts derived from the indicated genotypes were pretreated with IFN-β for 24 h. Cells were then infected with VSV-GFP (green) at an MOI of 0.01 and imaged after 24 h. Representative bright field and fluorescent images are shown. ( b ) Western blot analysis shows STAT2 expression in fibroblasts of indicated genotypes. Representative images are shown of n = 2.
Techniques Used: Derivative Assay, Infection, Western Blot, Expressing
Figure Legend Snippet: Efficient targeted deletion of Stat2 in mouse tissues. Expression of STAT2 and STAT1 was analyzed in various tissues by Western blot analysis. ( a ) Bone marrow–derived cDCs generated from Stat2 fl/fl mice crossed with CD11c-Cre mice ( Stat2 Δ-cDC ). ( b ) Colons from Stat2 fl/fl mice crossed with Cdx2-Cre mice ( Stat2 Δ-CE ). ( c ) Lungs from Stat2 Δ-cDC and Stat2 Δ-CE mice were included for specificity of targeted deletion. Wild-type (WT) and Stat2KO mice served as positive and negative controls, respectively. ACTIN was used as an internal protein loading control.
Techniques Used: Expressing, Western Blot, Derivative Assay, Generated, Control
Related Articles
Incubation:Article Title: Porcine reproductive and respiratory syndrome virus nsp5 inhibits the activation of the Nrf2/HO-1 pathway by targeting p62 to antagonize its antiviral activity Article Snippet: .. Membranes were blocked with 5% non-fat milk and incubated overnight at 4°C with specific primary antibodies: anti-PRRSV N antibody (GTX637947); anti-Nrf2 (T55136), anti-Flag-tag (M20008), anti-IRF3 (T55779), anti-p-IRF3 (TA2436), anti-STAT1 (T55227), anti-p-STAT1 (T55702), and anti-p-STAT3 (T56566) purchased from Abmart; anti-β-actin (66009), anti-HO-1 (10701), anti-Keap1 (10503), anti-p62 (18420), anti-p65 (10745), anti-p-p65 (82335), anti-HA-tag (51064), anti-Myc-tag (10828), Article Title: The main protease of SARS-CoV-2 cleaves histone deacetylases and DCP1A, attenuating the immune defense of the interferon-stimulated genes. Article Snippet: The following antibodies were used for immunoprecipitation analysis: Rb anti-HDAC2 (Cell Signaling, 57156), Ms antiHDAC2 (Santa Cruz, sc-9959), Rb anti-HDAC1 (Abcam, ab280198), Ms anti-HDAC1 (Cell Signaling, 5356), Rb antiHDAC3 (Proteintech, 10255-1-AP), and Ms anti-HDAC3 (Proteintech, 67151). .. HeLa cells were grown on glass bottom cell culture dish (NEST) and transfected with Mpro-mCherry construct, and after 24 h of expressing, cells were treated with 1000 U/ml IFNα for 1 h, then cells were fixed in 1 ml of 4% formaldehyde for 30 min and permeabilized in 0.5% TritonX-100 for 10 min. After washing with PBS, samples were blocked with 10% goat serum for 1 h and incubated with the anti-STAT1(Cell Signaling, 14994), Immunohistochemistry:Article Title: USP20-Driven Cholesterol Metabolism Links Inflammatory Signaling to Malignancy and Stromal Co-evolution in Pancreatic Cancer Article Snippet: 1 Title: USP20-Driven Cholesterol Metabolism Links Inflammatory Signaling to 1 Malignancy and Stromal Co-evolution in Pancreatic Cancer 2 Authors: 3 Xiangyan Jiang, 1,2,3# Bin Zhao, 1,2,3# Tao Wang, 1,2,4# Yong Ma, 1,2,4# Wenbo Liu, 1,2 Haonan Sun, 1,2 4 Zhigang Li, 5 Keshen Wang, 1,2 Qichen He, 1,2 Xiaoying Guan, 6 Long Qin, 3,4,7 Wengui Shi, 3,4,7 5 Yuman Dong, 3,4,7 Zhenzhen Ye, 8 Chengliang Zhou, 9 Xiaoe He, 1,2 Huiguo Qing, 1 Bo Long, 1,2 6 Huinian Zhou, 1,2 Zeyuan Yu, 2,4 Zuoyi Jiao 2,3,4,10* 7 Affiliations: 8 1 The Second Clinical Medical School, Lanzhou University, Lanzhou 730000, China.. 9 2 Department of General Surgery, Lanzhou University Second Hospital, Lanzhou 730000, 10 China.. 11 3 Gansu Tumor Immunology Basic Disciplines Research Center, Lanzhou University, Lanzhou 12 730000, China. Cell Culture:Article Title: The main protease of SARS-CoV-2 cleaves histone deacetylases and DCP1A, attenuating the immune defense of the interferon-stimulated genes. Article Snippet: The following antibodies were used for immunoprecipitation analysis: Rb anti-HDAC2 (Cell Signaling, 57156), Ms antiHDAC2 (Santa Cruz, sc-9959), Rb anti-HDAC1 (Abcam, ab280198), Ms anti-HDAC1 (Cell Signaling, 5356), Rb antiHDAC3 (Proteintech, 10255-1-AP), and Ms anti-HDAC3 (Proteintech, 67151). .. HeLa cells were grown on glass bottom cell culture dish (NEST) and transfected with Mpro-mCherry construct, and after 24 h of expressing, cells were treated with 1000 U/ml IFNα for 1 h, then cells were fixed in 1 ml of 4% formaldehyde for 30 min and permeabilized in 0.5% TritonX-100 for 10 min. After washing with PBS, samples were blocked with 10% goat serum for 1 h and incubated with the anti-STAT1(Cell Signaling, 14994), Transfection:Article Title: The main protease of SARS-CoV-2 cleaves histone deacetylases and DCP1A, attenuating the immune defense of the interferon-stimulated genes. Article Snippet: The following antibodies were used for immunoprecipitation analysis: Rb anti-HDAC2 (Cell Signaling, 57156), Ms antiHDAC2 (Santa Cruz, sc-9959), Rb anti-HDAC1 (Abcam, ab280198), Ms anti-HDAC1 (Cell Signaling, 5356), Rb antiHDAC3 (Proteintech, 10255-1-AP), and Ms anti-HDAC3 (Proteintech, 67151). .. HeLa cells were grown on glass bottom cell culture dish (NEST) and transfected with Mpro-mCherry construct, and after 24 h of expressing, cells were treated with 1000 U/ml IFNα for 1 h, then cells were fixed in 1 ml of 4% formaldehyde for 30 min and permeabilized in 0.5% TritonX-100 for 10 min. After washing with PBS, samples were blocked with 10% goat serum for 1 h and incubated with the anti-STAT1(Cell Signaling, 14994), Construct:Article Title: The main protease of SARS-CoV-2 cleaves histone deacetylases and DCP1A, attenuating the immune defense of the interferon-stimulated genes. Article Snippet: The following antibodies were used for immunoprecipitation analysis: Rb anti-HDAC2 (Cell Signaling, 57156), Ms antiHDAC2 (Santa Cruz, sc-9959), Rb anti-HDAC1 (Abcam, ab280198), Ms anti-HDAC1 (Cell Signaling, 5356), Rb antiHDAC3 (Proteintech, 10255-1-AP), and Ms anti-HDAC3 (Proteintech, 67151). .. HeLa cells were grown on glass bottom cell culture dish (NEST) and transfected with Mpro-mCherry construct, and after 24 h of expressing, cells were treated with 1000 U/ml IFNα for 1 h, then cells were fixed in 1 ml of 4% formaldehyde for 30 min and permeabilized in 0.5% TritonX-100 for 10 min. After washing with PBS, samples were blocked with 10% goat serum for 1 h and incubated with the anti-STAT1(Cell Signaling, 14994), Expressing:Article Title: The main protease of SARS-CoV-2 cleaves histone deacetylases and DCP1A, attenuating the immune defense of the interferon-stimulated genes. Article Snippet: The following antibodies were used for immunoprecipitation analysis: Rb anti-HDAC2 (Cell Signaling, 57156), Ms antiHDAC2 (Santa Cruz, sc-9959), Rb anti-HDAC1 (Abcam, ab280198), Ms anti-HDAC1 (Cell Signaling, 5356), Rb antiHDAC3 (Proteintech, 10255-1-AP), and Ms anti-HDAC3 (Proteintech, 67151). .. HeLa cells were grown on glass bottom cell culture dish (NEST) and transfected with Mpro-mCherry construct, and after 24 h of expressing, cells were treated with 1000 U/ml IFNα for 1 h, then cells were fixed in 1 ml of 4% formaldehyde for 30 min and permeabilized in 0.5% TritonX-100 for 10 min. After washing with PBS, samples were blocked with 10% goat serum for 1 h and incubated with the anti-STAT1(Cell Signaling, 14994), |